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  • Title: Differential expression of serotonin and [Met]enkephalin-Arg6-Gly7-Leu8 in neurons of the rat brain stem.
    Author: Tanaka M, Okamura H, Yanaihara N, Tanaka Y, Ibata Y.
    Journal: Brain Res Bull; 1993; 30(5-6):561-70. PubMed ID: 8457905.
    Abstract:
    The hypothesis that serotonin (5-HT) and [Met]enkephalin-Arg6-Gly7-Leu8 (MEAGL) coexist in the rat brain stem raphe nuclei was tested by a technique combining histofluorescence with immunocytochemistry, after treatment with colchicine and nialamide. In midbrain and pons serotonergic cell groups (B5-B9), no coexistence of 5-HT and MEAGL was detected. In serotonergic cell groups of the medulla oblongata (B1-B3), only 0.3-1.5% of 5-HT-fluorescent cells were MEAGL-immunoreactive. These findings suggest that putative 5-HT and MEAGL are mostly expressed in different populations of neurons in the rat raphe nuclei.
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