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  • Title: A rapid fluorometric method for the determination of carnitine palmitoyltransferase.
    Author: Schäfer J, Turnbull DM, Reichmann H.
    Journal: Anal Biochem; 1993 Feb 15; 209(1):53-6. PubMed ID: 8465961.
    Abstract:
    A rapid and sensitive fluorometric assay for the measurement of carnitine palmitoyltransferase (CPT) activity is described. In this assay the coenzyme A (CoA) liberated from palmitoyl-CoA by CPT reacts with N-(9-acridinyl)-maleimide to form a fluorescent product. By comparing the fluorescence intensities of external CoA standards, CPT activity can be determined. The coefficients of variation within-run and between-run of the method were 6.9 and 10%, respectively. The CPT activity of human muscle samples measured with this assay is comparable to those obtained with a commonly used radiochemical assay and CPT deficiency in skeletal muscle from a patient was detected by this new fluorometric assay.
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