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  • Title: Regulation of the rat class I alcohol dehydrogenase gene by growth hormone.
    Author: Potter JJ, Yang VW, Mezey E.
    Journal: Biochem Biophys Res Commun; 1993 Mar 31; 191(3):1040-5. PubMed ID: 8466483.
    Abstract:
    Growth hormone (GH) increases the transcription of the rat class I alcohol dehydrogenase (ADH) gene. Two transcription factors, the CCAAT/enhancer binding protein (C/EBP) and the liver activator protein (LAP), were previously shown to bind to the ADH promoter at nucleotide positions -11 to -22 relative to the start-site of transcription and to activate the ADH promoter in co-transfection experiments. In this study, exposure of cultured rat hepatocytes to GH (1 micrograms/ml) for 4 days increased LAP mRNA, but not C/EBP mRNA, in conjunction with an increase in ADH mRNA. GH, in transient transfection experiments of primary rat hepatocyte cultures, activated an ADH promoter-reporter gene construct containing the C/EBP binding site, but failed to activate a construct containing a 4-bp mutation at this site. These results suggest that the effect of GH in enhancing ADH promoter activity is mediated by LAP binding to the C/EBP site.
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