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  • Title: Carbon dioxide anesthesia in phlebotomine sand flies (Diptera: Psychodidae): CO2 effect upon two laboratory colonies.
    Author: Perrotti E, Maroli M.
    Journal: J Am Mosq Control Assoc; 1993 Mar; 9(1):94-6. PubMed ID: 8468582.
    Abstract:
    Gravid females of 2 sand fly species, Phlebotomus papatasi and P. perniciosus, were exposed to carbon dioxide anesthesia for 5, 10 and 20 minutes. Recovery time, mortality at 0 min and 24 h, percentage of females laying eggs, time to oviposition, and egg productivity for each exposure time were registered. Survival, fecundity and oviposition time in the 2 species were not adversely affected by the short period of CO2 anesthesia routinely used in the sand fly colony maintenance.
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