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  • Title: The Ti plasmid from the wide host range Agrobacterium vitis strain Tm4: map and homology with other Ti plasmids.
    Author: Otten L, Gérard JC, De Ruffray P.
    Journal: Plasmid; 1993 Mar; 29(2):154-9. PubMed ID: 8469721.
    Abstract:
    The octopine/cucumopine (o/c) strains of Agrobacterium vitis are divided into limited (LHR) and wide host range (WHR) strains. We present here the first map of a Ti plasmid from a WHR o/c strain, Tm4. pTiTm4 (252 kb) has homology with the octopine Ti plasmid pTiAch5, the nopaline plasmid pTiC58, and the Ri plasmid pRiHRI. Approximately 155 kb of pTiTm4 is homologous with the LHR o/c Ti plasmids pTiAB3 and pTiAg57, the remaining part is pTiTm4-specific. Our data suggest that the evolution of the o/c Ti plasmids was accompanied by large-scale changes in plasmid structure. The origin of replication of pTiTm4 was localized by a functional assay.
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