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  • Title: False negative biopsy in Pneumocystis carinii pneumonia.
    Author: Demicco WA, Stein A, Urbanetti JS, Fanburg BL.
    Journal: Chest; 1979 Mar; 75(3):389-90. PubMed ID: 84733.
    Abstract:
    Unlike most pneumonias, the diagnosis of Pneumocystis carinii pneumonia is based solely on identifying organisms by stain, usually with methenamine-silver. Because of technical problems involved with adequate staining, control samples usually are done concurrent with tissue specimens to be examined. Lung containing fungi often is used as a control. We recently observed false-negative biopsy specimens in a case of P carinii pneumonia where the Pneumocystis organism failed to stain with methenamine-silver on several occasions, although fungal controls were positive. This report emphasizes the importance of using P carinii as a control whenever attempting to diagnose P carinii pneumonia.
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