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  • Title: A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids.
    Author: Keherly MJ, Papaconstantinou J, McCombs JL.
    Journal: Exp Cell Res; 1993 Apr; 205(2):361-4. PubMed ID: 8482340.
    Abstract:
    We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.
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