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  • Title: Identification and immunohistochemistry of retinol dehydrogenase from bovine retinal pigment epithelium.
    Author: Suzuki Y, Ishiguro S, Tamai M.
    Journal: Biochim Biophys Acta; 1993 May 13; 1163(2):201-8. PubMed ID: 8490052.
    Abstract:
    We studied the properties of retinol dehydrogenase (11-cis-specific) from bovine retinal pigment epithelium. Detergents caused a loss of retinol dehydrogenase activity; therefore, we added 3 mM NADH as a stabilizer to solubilize this enzyme and partially purified this enzyme using Sepharose CL-6B and hydroxyapatite column chromatography. The partially-purified sample, which contained two major proteins (66 kDa, 33 kDa), had substrate preference to 11-cis and 13-cis-retinal but not to all-trans and 9-cis isomers. Monoclonal anti-33 kDa protein of retinal pigment epithelial crude extract by Western blotting. In addition, we found that monoclonal anti-retinol dehydrogenase antibody bound specifically to retinal pigment epithelium and not to Müller cells or to rod outer segments by immunohistochemical methods.
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