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  • Title: Application of cryotechniques in cartilage tissue preservation and immunoelectron microscopy: potentials and problems.
    Author: Hunziker EB.
    Journal: Microsc Res Tech; 1993 Apr 15; 24(6):457-64. PubMed ID: 8490231.
    Abstract:
    Cryotechnical processing of cartilage has the potential to solve many of the tissue-specific problems associated with various routine chemical fixation protocols. This is particularly the case with respect to extracellular matrix architecture, the distortion or destruction of which (caused by extraction and/or precipitation of proteoglycan molecules) may be prevented. Adoption of such techniques also permits high-sensitivity immunoelectron-microscopy of the extracellular matrix space (carbohydrate epitopes). However, a number of difficulties still remain to be resolved, particularly that of matrix-cell interface separation occurring during freeze substitution and low temperature embedding. These problems are briefly addressed and possible solutions outlined.
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