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  • Title: Solubilization of active somatostatin receptors from rabbit retina.
    Author: Liapakis G, Politou E, Thermos K.
    Journal: Biochem Pharmacol; 1993 May 05; 45(9):1821-8. PubMed ID: 8494540.
    Abstract:
    Somatostatin receptors from rabbit retinal membranes were solubilized in an active form using a mixture of the detergent n-octyl b-D-glucopyranoside (OG) and CHAPS. The binding of [125I]-Try11-somatostatin to the soluble extract was saturable and of high affinity, with an apparent affinity constant (Kd) of 0.60 +/- 0.20 nM and a maximum number of binding sites (Bmax) of 80 +/- 48 fmol/mg protein. The specific binding of [125I]Tyr11-somatostatin was inhibited in a dose-dependent manner only by the somatostatinergic analogs. The biochemical characteristics of both the membrane-bound and soluble receptors were studied by photoaffinity labeling techniques. Analysis by SDS-PAGE and subsequent autoradiography revealed the presence of a major protein of similar relative molecular mass (M(r) 54,000 and 57,000 for membrane and soluble sites, respectively). The photolabeling of this protein was specifically inhibited by somatostatin-28, somatostatin-14, SMS 201-995 (a synthetic octapeptide analog of somatostatin) but not by bombesin and somatostatin-28(1-14). The non-hydrolysable GTP analog guanosine-5'-O-(3-thio-triphosphate) (GTP gamma S) regulated the photolabeling of [125I]Tyr11-somatostatin to the membrane and soluble receptors. These studies describe for the first time the successful solubilization of the somatostatin receptor and the biochemical characterization of both membrane-bound and soluble receptors from rabbit retina.
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