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  • Title: [Preparation and bacterial expression of a mutant gene for human lymphotoxin].
    Author: Korobko VG, Davydov IV, Dobrynin VN, Pustoshilova NM, Lebedev LR, Gileva IP, Petrenko VA.
    Journal: Bioorg Khim; 1993 Apr; 19(4):414-9. PubMed ID: 8494564.
    Abstract:
    Using the oligonucleotide directed mutagenesis, a human lymphotoxin (TNF beta) mutant gene lacking 21 N-terminal codons has been obtained. Recombinant plasmid pLT21 for expression of the mutant gene has been constructed. The mutant gene in the plasmid was placed under control of a tandem of constitutive promoters from coliphage T7. A simple procedure for isolation of recombinant protein was developed. The procedure allows to obtain the highly purified biologically active mutant protein with a good yield. During biosynthesis the recombinant protein undergoes a posttranslational processing resulted in the cleavage of N-terminal methionine and leucine residues.
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