These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Human naive T cells are preferentially stimulated by crosslinking of CD3 and CD45RA with monoclonal antibodies.
    Author: Welge T, Wolf M, Jäggle C, Luckenbach GA.
    Journal: Cell Immunol; 1993 Apr 15; 148(1):218-25. PubMed ID: 8495489.
    Abstract:
    To analyze the role of CD45 molecules in CD3-mediated activation of T cells, we analyzed the effect of crosslinking different CD45 isoforms with mAbs on the proliferation of various T cell subsets in vitro. Crosslinking of CD3 and CD45RA molecules with the mAb 2H4 or WR16 resulted in the preferential stimulation of enriched naive (CD45RA+) T cells, whereas crosslinking of CD3 alone led to stimulation of enriched memory (CD45RO+) T cells. In contrast, proliferation of memory T cells was not enhanced by additional crosslinking with the memory T cell marker CD45RO. To induce the costimulatory effect on naive T cells, an intense crosslinking of the TcR/CD3 complex and CD45RA molecules by immobilized secondary antibody is necessary because enhanced proliferation did not occur when the antibodies were directly immobilized. The same differences in reactivity of CD45RA-enriched naive and CD45RO-enriched memory T cell subset could be shown by using a mAb to common CD45, indicating that the effects are not mediated by a particular antibody or by binding to different epitopes. The CD45RA-induced differences in proliferation of naive and memory T cells could not be abolished by the addition of exogenous IL2. In contrast, naive T cells were more responsive to exogenous IL2 than memory T cells independently of CD45RA crosslinking, indicating that IL2 is not responsible for the observed differences in T cell proliferation.
    [Abstract] [Full Text] [Related] [New Search]