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  • Title: Ion-pair extraction of histamine from biological fluids and tissues for its determination by high-performance liquid chromatography with fluorescence detection.
    Author: Tsikas D, Velásquez R, Toledano C, Brunner G.
    Journal: J Chromatogr; 1993 Apr 21; 614(1):37-41. PubMed ID: 8496284.
    Abstract:
    A procedure for the selective ion-pair extraction of histamine from various biological fluids and tissues by using the ion-pairing agent bis(2-ethylhexyl)hydrogen-phosphoric acid has been developed. This procedure enabled routine determination of histamine in plasma, urine and tissue samples of humans and rats by reversed-phase high-performance liquid chromatography with fluorescence detection using o-phthaldialdehyde.
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