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Title: Purification and crystallization of fumarase C from Escherichia coli. Author: Weaver TM, Levitt DG, Banaszak LJ. Journal: J Mol Biol; 1993 May 05; 231(1):141-4. PubMed ID: 8496960. Abstract: Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C222(1) form is complete to 2.10 A and the I222 form is complete to 2.20 A.[Abstract] [Full Text] [Related] [New Search]