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  • Title: Single step purification of platelet factor XIII using an immobilized factor XIII a-subunit monoclonal antibody.
    Author: Lukacova D, Reed GL.
    Journal: Thromb Haemost; 1993 Apr 01; 69(4):397-400. PubMed ID: 8497854.
    Abstract:
    To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.
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