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  • Title: The salvage of deoxycytidine into dCDP-diacylglycerol by macrophages and lymphocytes.
    Author: Hrabák A, Spasokukotskaja T, Temesi A, Staub M.
    Journal: Biochem Biophys Res Commun; 1993 May 28; 193(1):212-9. PubMed ID: 8503910.
    Abstract:
    Extracellular deoxycytidine (CdR) was previously shown to be salvaged into water soluble [1] and also into lipidic [2] precursors of phospholipids in stimulated lymphocytes and in lymphoma cells [3]. In this paper we have described that non-dividing murine macrophages salvaged not only 5-3H-CdR but also tritiated thymidine (3H-TdR) mainly into the pools as nucleotides. Chlorpromazine shifted the CdR salvage into a lipidic compound of the cells which was identified as 3H-dCDP-diacylglycerol (dCDP-DAG). After 5-3H-CdR labeling the lipid/DNA ratio was eleven times higher in macrophages than in tonsillar lymphocytes. Thin layer chromatography (TLC) on borate impregnated silica gel plates gave clear separation of CDP-DAG from dCDP-DAG supporting that the extracellular precursor for it is exclusively deoxycytidine and not ribocytidine. No interconversion between deoxy- and and ribocytidine could be observed neither in lymphocytes nor in macrophages.
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