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  • Title: Effects of protein kinase C activation on intracellular Ca2+ distribution in vascular smooth muscle cells of spontaneously hypertensive rats.
    Author: Neusser M, Golinski P, Zhu Z, Tepel M, Zidek W.
    Journal: J Vasc Res; 1993; 30(2):116-20. PubMed ID: 8504195.
    Abstract:
    Protein kinase C is known to influence contraction in vascular smooth muscle cells by Ca(2+)-dependent and Ca(2+)-independent mechanisms. In the present study, the effect of protein kinase C activation by phorbol 12-myristate 13-acetate on resting cytosolic free Ca2+ and on cellular Ca2+ pools was assessed in cultured rat aortic muscle cells using fura 2. Cellular Ca2+ pools were evaluated with the selective inhibitor of the sarcoplasmic Ca2+ ATPase, thapsigargin. In normotensive vascular smooth muscle cells, protein kinase C activation caused a redistribution of Ca2+ from the thapsigargin-sensitive pool into the cytoplasm, whereas, in hypertensive cells, no significant effect of protein kinase C activity on cellular Ca2+ distribution was found. It is concluded that protein kinase C modulates the amount of Ca2+ stored in the thapsigargin-sensitive calcium stores. In hypertensive cells, the regulation of Ca2+ pools by protein kinase C is disturbed.
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