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  • Title: Methods for measurement of antibody/antigen affinity based on ELISA and RIA.
    Author: Goldberg ME, Djavadi-Ohaniance L.
    Journal: Curr Opin Immunol; 1993 Apr; 5(2):278-81. PubMed ID: 8507406.
    Abstract:
    Various enzyme-linked immunosorbent assay or radioimmunoassay methods are currently used to quantify the antibody-antigen interaction. Only those based on indirect competition--enzyme-linked immunosorbent assay or radioimmunoassay--can provide the real thermodynamic affinity of the antibody for its antigen. They can be applied to a variety of experimental situations, some of which are reviewed here.
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