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  • Title: [Synthesis, cloning, and determination of the primary structure of a full-length DNA copy of the gene for influenza A/Alma-Ata/1417/84 virus (H1N1-serovariant HSW1N1) hemagglutinin].
    Author: Beklemishev AB, Nazarova LM, Filimonov NG, Blinov VM, Grinev AA, Chuvakova ZK, Kim EV, Mukazhanova GN.
    Journal: Mol Gen Mikrobiol Virusol; 1993; (1):24-7. PubMed ID: 8510679.
    Abstract:
    The full-length copy of the hemagglutinin gene RNA of the influenza virus A/Alma Ata/1417/84 (Hsw1 N1-serovariant) has been synthesized and cloned on Escherichia coli plasmid pBR327. The complete nucleotide sequence of the cloned DNA copy was determined by the Maxam-Gilbert procedure. The predicted amino acid, sequence of HA1 hemagglutinin subunit was compared with the sequences of HA1 subunits from other H1N1-subtype influenza virus strains. It has been found that the structure of the HA1-subunit of the studied strain is most similar to the structure of the identical region of the A/New Jersey/18/76 hemagglutinin.
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