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  • Title: Immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants.
    Author: Ravelonandro M, Peyruchaud O, Garrigue L, de Marcillac G, Dunez J.
    Journal: Arch Virol; 1993; 130(3-4):251-68. PubMed ID: 8517789.
    Abstract:
    Tobacco plants (Nicotiana tabacum cv. Xanthi) have been transformed via Agrobacterium tumefaciens vectors, with cDNAs corresponding to the plum pox virus (PPV) cistron 2 encoding helper component (HC-Pro) and with the first two and half cistrons of the PPV genome. Presence of the HC-Pro in PPV-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the PPV HC-Pro. The results suggest that two proteases are involved in the processing of the PPV N-terminal polyprotein to yield a protein of 48 k (HC-Pro). HC-Pro autolytically cleaves at its carboxyl-terminus and a proteolytic activity, probably associated with the protein (P1) encoded by the cistron 1, is required for the cleavage in planta between the proteins derived from cistrons 1 and 2.
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