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  • Title: Purification and characterization of a bone-inducing protein from a murine osteosarcoma (Dunn type).
    Author: Takaoka K, Yoshikawa H, Hashimoto J, Miyamoto S, Masuhara K, Nakahara H, Matsui M, Ono K.
    Journal: Clin Orthop Relat Res; 1993 Jul; (292):329-36. PubMed ID: 8519128.
    Abstract:
    A protein fraction capable of eliciting cartilage or bone formation in vivo was purified more than 100,000-fold from a murine osteosarcoma (Dunn type). Intramuscular implantation of as little as 20 ng of the purified protein with 2 mg of pure skin collagen consistently induced ectopic new bone formation. The apparent molecular size of the purified protein was 32 kd on sodium dodecylsulfate-polyacrylamide gel electrophoresis. On reduction, the 32-kd protein split into subunits with the same partial amino acid sequences, and these partial sequences were identical to those of human bone morphogenetic protein-2B (BMP-4) which is assumed to be a member of the transforming growth factor-beta superfamily.
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