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  • Title: Serological survey of canine distemper virus infection using enzyme-linked immunosorbent assay.
    Author: Gemma T, Miyashita N, Shin YS, Okita M, Mori T, Iwatsuki K, Mikami T, Kai C.
    Journal: J Vet Med Sci; 1995 Aug; 57(4):761-3. PubMed ID: 8519915.
    Abstract:
    For detection of antibodies against canine distemper virus (CDV), an enzyme-linked immunosorbent assay (ELISA) was developed using a crude extract from cells infected with the Onderstepoort strain of CDV as antigen. Twenty-six sera from dogs experimentally vaccinated with the Snyder-Hill strain of CDV were compared by ELISA and a standard virus neutralization (NV) test. Since a good correlation between the titers obtained by both tests was observed, ELISA was considered to be a rapid and reliable method for a serological survey of CDV infection. When a total of 167 sera from dogs suspected of canine distemper under natural conditions were examined by the ELISA, 29 of the sera (17%) were found to have low VN antibody titers and high ELISA titers. The reason for the discrepancy in the titers was discussed.
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