These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Cloning, sequencing and expression of the gene encoding a major allotypic preprocarboxypeptidase A from bovine pancreas.
    Author: Goo JH, Kim KH, Choi KY.
    Journal: Gene; 1995 Nov 20; 165(2):333-4. PubMed ID: 8522204.
    Abstract:
    A cDNA encoding one of two major allotypic forms of bovine pancreatic preprocarboxypeptidase A (preproCPA) has been cloned, and its entire nucleotide (nt) sequence determined. The cloned cDNA contains a 26-nt 5'-noncoding region, a 1260-nt open reading frame and a 51-nt 3'-noncoding region. The amino acid (aa) sequence deduced from the gene sequence contains Ile179, Ala228 and Val305 at allotypic aa residues. The procarboxypeptidase A (proCPA) was produced in yeast and secreted into the medium. Upon treatment with trypsin, proCPA generated an active enzyme with the same size as the mature carboxypeptidase A (CPA), as analyzed by use of anti-CPA polyclonal antibody.
    [Abstract] [Full Text] [Related] [New Search]