These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of essential fatty acid deficiency on forskolin binding sites, adenylate cyclase and cyclic AMP-dependent protein kinase activity, the levels of G proteins and ventricular function in rat heart.
    Author: Alam SQ, Mannino SJ, Alam BS, McDonough K.
    Journal: J Mol Cell Cardiol; 1995 Aug; 27(8):1593-604. PubMed ID: 8523422.
    Abstract:
    Three groups of rats were fed semi-purified diets. Diet I was deficient in essential fatty acids (EFAD), diet II was marginally deficient in essential fatty acids (MEFAD), and diet III contained adequate levels of essential fatty acids (control). After 9 weeks, some rats within each group were killed and cardiac membranes were prepared. Adenylate cyclase activity, [3H]forskolin binding sites, the levels of G proteins (Gi and Gs) and fatty acid composition of the membrane phospholipids were measured. Typical changes of EFA deficiency were observed in fatty acid composition of the membrane phospholipids. Adenylate cyclase activity was significantly lower in membranes of EFA-deficient rats than those of the controls. The MEFAD group gave intermediate values. Similar results were obtained with forskolin-stimulated activity with different concentrations of forskolin. Concentrations of the forskolin binding sites were also lower in the EFAD, but not in the MEFAD rats compared with the controls. There was no significant difference in forskolin binding affinities among the three groups. The decrease in adenylate cyclase activity in EFA-deficient rat heart was partially restored by feeding the control diet for 5 weeks to the EFAD or the MEFAD rats. The levels of Gi alpha and Gs alpha were not significantly different in cardiac membranes of rats fed the EFAD or the MEFAD diets from those of the control group. Lower adenylate cyclase activity in hearts of EFAD rats was also reflected in correspondingly lower activity of cAMP-dependent protein kinase. The results suggest an impairment of the cellular signalling pathway for the production of cAMP in rat heart during EFA deficiency. The beta-adrenergic response of isolated heart preparations obtained from rats fed the three diets was, however, not significantly altered.
    [Abstract] [Full Text] [Related] [New Search]