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  • Title: Characterization of human DNA ligase I expressed in a baculovirus-insect cell system.
    Author: Gallina A, Rossi F, Milanesi G, Rossi R, Montecucco A, Ciarrocchi G.
    Journal: Biochem J; 1995 Dec 01; 312 ( Pt 2)(Pt 2):593-7. PubMed ID: 8526875.
    Abstract:
    The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 10(8) Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein.
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