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  • Title: Detection of MspI RFLP in human THY1 gene by the polymerase chain reaction.
    Author: Fu J, Ikegami H, Yamato E, Kawaguchi Y, Takekawa K, Fujisawa T, Nakagawa Y, Hamada Y, Ueda H, Shen GQ.
    Journal: Jpn J Hum Genet; 1995 Sep; 40(3):279-80. PubMed ID: 8527803.
    Abstract:
    THY1 gene encodes a cell surface glycoprotein predominantly expressed in brain and peripheral nerves. Human THY1 gene region on chromosome 11q23 has been implicated in susceptibility to type 1 diabetes (Wong et al., 1991). Two primers derived from the sequences flanking the polymorphic MspI site in intron 2 of the human THY1 gene (Gatti et al., 1988) were selected for RCP to amplify a 566 bp fragment that spans the MspI polymorphism. Polymorphism was detected by MspI digestion of the PCR product.
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