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  • Title: Functional domain mapping using M13 deletions.
    Author: Grammatikakis N, Toole BP.
    Journal: Biochem Mol Biol Int; 1995 Jul; 36(4):771-9. PubMed ID: 8528139.
    Abstract:
    We describe a convenient approach for concomitant functional characterization of peptide domains (monoclonal antibody epitopes, receptor-ligand, DNA-protein, and protein-protein interaction sites, etc) encoded by the sequential series of overlapping M13 subclones generated for nucleotide sequence determination of a new cDNA. We have employed this method to rapidly map the location and amino acid sequence of an epitope-containing domain within a polypeptide encoded by a newly isolated cDNA.
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