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  • Title: Biochemical applications of a quantitative high-pressure liquid chromatographic assay of warfarin and its metabolites.
    Author: Fasco MJ, Piper LJ, Kaminsky LS.
    Journal: J Chromatogr; 1977 Jan 21; 131():365-73. PubMed ID: 853101.
    Abstract:
    A quantitative high-pressure liquid chromatographic assay of warfarin and its diastereoisomeric alcohols and 4'-, 6-, 7-, 8- and benzylic hydroxylated metabolites was accomplished using a reversed-phase, microparticle column (muBondapak/C18) employing 1.5% acetic acid (pH 4.7)-acetonitrile (69:31) as solvent. Concentration of these compounds on the column prior to their elution permitted the loading of 0.01-0.5 ml solutions of warfarin and metabolites without consequent alterations in peak heights and resolution. Details of the use of this technique in the study of R and S warfarin metabolism by hepatic mixed function oxidases and in the determination of warfarin and its metabolites in blood are presented.
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