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  • Title: A computer program for predicting recovery of SDS-protein in the automated HPGE-1000 apparatus.
    Author: Aldroubi A, Zakharov SF, Chrambach A.
    Journal: Appl Theor Electrophor; 1995; 5(1):31-4. PubMed ID: 8534752.
    Abstract:
    The commercial automated gel electrophoresis apparatus (HPGE-1000 of LabIntelligence, Menlo Park, CA) allows one to recover the material migrating and visualized as a fluorescent-labeled band by electrophoresis into a collection cup located above the band at a right angle to the orientation of the separation path. The degree of recovery is a function of sample load (peak area), electrophoresis time at constant field strength, the mobility of the material and band width. Neglecting the latter, recovery of several SDS-proteins was measured as a function of the first three parameters. These measurements were used as a data base for a computer program capable of predicting, by interpolation of the experimental values, the time of electrophoresis needed to obtain a specified degree of recovery, or the degree of recovery obtained after a desired time of electrophoresis into the collection cup.
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