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  • Title: Molecular cloning, characterization and expression of an elongation factor 1 alpha gene in maize.
    Author: Berberich T, Sugawara K, Harada M, Kusano T.
    Journal: Plant Mol Biol; 1995 Nov; 29(3):611-5. PubMed ID: 8534856.
    Abstract:
    A cDNA (zmEF1A) and the corresponding genomic clone (zmgEF1A) of a member of the gene family encoding the alpha subunit of translation elongation factor 1 (EF-1 alpha) have been isolated from maize. The deduced amino acid sequence is 447 residues long interrupted by one intron. Southern blot analysis reveals that the cloned EF-1 alpha gene is one member out of a family consisting of at least six genes. As shown by northern hybridizations in leaves the mRNA level increases at low temperature whereas time-course experiments over 24 h at 5 degrees C show that in roots the overall mRNA level of EF-1 alpha is transiently decreased. These results indicate that the expression of EF-1 alpha is differently regulated in leaves and roots under cold stress.
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