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  • Title: On the cell-free system of protein synthesis from mammalian mitochondria.
    Author: Kisselev OI, Gaitskhoki VS, Neifakh SA.
    Journal: Mol Cell Biochem; 1977 Feb 04; 14(1-3):91-6. PubMed ID: 854030.
    Abstract:
    Some properties of a submitochondrial cell-free system for protein synthesis are described. The system was prepared from rat liver mitochondria lysed with Triton X-100, and the lysate was characterized by a linear rate of [14C]amino acid incorporation for 15-20 min with subsequent decline in activity. The incorporation reaction was inhibited by chloramphenicol and was insensitive to cycloheximide. Poly(U) addition stimulated [14CA1phenylalanine incorporation by the preincubated submitochondrial system. Upon the addition of 7.5S mRNA that was isolated from mitochondria the major translation product was identified as a hydrophobic poly-peptide which in some properties (solubility in chloroform-methanol mixture) was similar to one of polypeptides synthesized by the sub-mitochondrial system on endogeneous mRNAs.
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