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  • Title: Deletion of the 3' splice site of the leader-variable region intron of immunoglobulin heavy chain genes induces a direct splicing of leader to constant region, resulting in the production of truncated mu-chains.
    Author: Komori T, Sugiyama H.
    Journal: Eur J Immunogenet; 1995 Jun; 22(3):241-7. PubMed ID: 8547230.
    Abstract:
    An Abelson virus-transformed immature B cell line, AT8-1-12-5-2, produced truncated mu-chains. Sequencing analysis of the VHDJH complex on the expressed H-chain allele revealed the deletion of 75 nucleotides that involved leader-variable region intron and the 5' end of the variable region, which resulted in the loss of the 3' splice site of leader-variable region intron. Sequence studies of a leader- and CH1-containing cDNA clone showed that leader region was directly spliced to the CH1 exon, resulting in the production of the truncated mu-chains without variable portion. Our results demonstrated for the first time that the only loss of the 3' splice site of leader-variable region intron could induce an aberrant splicing between leader and constant region.
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