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  • Title: Determination of vitamin K1 in serum using catalytic-reduction liquid chromatography with fluorescence detection.
    Author: MacCrehan WA, Schönberger E.
    Journal: J Chromatogr B Biomed Appl; 1995 Aug 18; 670(2):209-17. PubMed ID: 8548011.
    Abstract:
    A new method for the liquid chromatographic-fluorescence determination of serum vitamin K1 is described using reduction of the K-quinone to the fluorescent K-hydroquinone. The reduction reaction occurs "on-line" in the LC system using a catalytic reducer column and an alcohol mobile phase as reductant. A procedure for serum determination utilizes a liquid-liquid serum lipid extraction followed by normal-phase fractionation on a solid-phase extraction cartridge. The final measurement uses a reversed-phase (C18) separation with a ethanol-methanol mobile phase and provides a detection limit of approximately 20 pg/ml.
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