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  • Title: Cattle serologically positive for Brucella abortus have antibodies to B. abortus Cu-Zn superoxide dismutase.
    Author: Tabatabai LB, Hennager SG.
    Journal: Clin Diagn Lab Immunol; 1994 Sep; 1(5):506-10. PubMed ID: 8556493.
    Abstract:
    In this study, we demonstrated by a Cu-Zn superoxide dismutase-specific enzyme-linked immunoassay that cattle that are serologically positive for Brucella abortus have serum immunoglobulin G antibodies to B. abortus Cu-Zn superoxide dismutase. The specificity of the antibody reactivity was confirmed by Western blot (immunoblot) analysis with B. abortus salt-extractable proteins containing native Cu-Zn superoxide dismutase and with recombinant B. abortus Cu-Zn superoxide dismutase. The results represent a first step in the direction of the development of a multiprotein diagnostic reagent for bovine brucellosis.
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