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  • Title: Search for antibodies for immunoenzymatic assay of human lutropin.
    Author: Szewczuk A, Kuropatwa M, Kochanowska I, Majewska A, Maćkiewicz Z, Kupryszewski G, Rapak A.
    Journal: Arch Immunol Ther Exp (Warsz); 1994; 42(5-6):453-8. PubMed ID: 8572906.
    Abstract:
    Five peptides corresponding to human lutropin (hLH) subunit fragments were synthesized by a solid phase method and their physicochemical characteristics are presented. Antibodies induced in rabbits with 3 peptides of beta hLH fragments coupled to thyrotropin did not bind hLH and human chorionic gonadotropin (hCG). Also 2 synthetic peptides of alpha hLH fragments did not react with rabbit anti-hLH, anti-hCG and anti-alpha hCG antibodies. Using 2 monoclonal anti-alpha hCG and anti-beta hLH antibodies a sensitive sandwich ELISA technique was elaborated. Using this technique stability of hLH was investigated. The ELISA was also applied for assays of urine hLH in normal and ovulation days.
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