These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Atrial natriuretic factor (ANF) prohormone accumulates in the IgG fraction of anti-ANF hybridoma-bearing mice.
    Author: Milne RW, Gutkowska J.
    Journal: Biochem Biophys Res Commun; 1996 Jan 26; 218(3):665-9. PubMed ID: 8579571.
    Abstract:
    We demonstrate immunoreactive mouse atrial natriuretic factor (ANF) in the IgG fraction prepared from ascites or serum of mice bearing an anti-rat ANFArg101-Tyr126 hybridoma. The HPLC profile of the ANF demonstrates that the IgG fraction contains both mature ANFSer99-Tyr126 and the prohormone ANFAsn1-Tyr126. The presence of circulating prohormone in the hybridoma-bearing mice is not secondary to changes in fluid volume due to ascites formation. The anti-ANF antibody appears to protect the ANFAsnl-Tyr126 from both maturation and rapid receptor-mediated clearance. Our results suggest, therefore, that the ANFAsn1-Tyr126 can be secreted into a compartment that is accessible to antibody and that maturation of the ANF prohormone may be an extracellular event.
    [Abstract] [Full Text] [Related] [New Search]