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  • Title: Detection of different quasispecies of hepatitis C virus core region in cancerous and noncancerous lesions.
    Author: Horie C, Iwahana H, Horie T, Shimizu I, Yoshimoto K, Yogita S, Tashiro S, Ito S, Itakura M.
    Journal: Biochem Biophys Res Commun; 1996 Jan 26; 218(3):674-81. PubMed ID: 8579573.
    Abstract:
    The quasispecies of hepatitis C virus (HCV) core region in non-cancerous and cancerous hepatocellular carcinoma (HCC) lesions, respectively, of 7 patients with hepatocellular carcinoma were studied. Multiple fluorescence based-polymerase chain reaction (PCR)-single strand conformation polymorphism exhibited a different set and a larger number of quasispecies in cancerous portions than those in non-cancerous portions. DNA sequencing of PCR-amplified core region substantiated an accumulation of nucleotide substitutions, and a greater number of quasispecies in cancerous portions than those in non-cancerous portions. The deduced amino acid sequences disclosed that at the peptide position 45, Ser is dominant in non-cancerous lesions, and Gly in cancerous lesions, respectively. Thus, HCV in hepatocellular carcinoma includes a large number of specific quasispecies presumably due to their vigorous proliferation. A different set of quasispecies with the amino acid change is presumed to be related to the hepatocarcinogenesis.
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