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  • Title: Increase of empty pool-activated Ca2+ influx using an intracellular Ca2+ chelating agent.
    Author: Skutella M, Rüegg UT.
    Journal: Biochem Biophys Res Commun; 1996 Jan 26; 218(3):837-41. PubMed ID: 8579601.
    Abstract:
    We demonstrate here that stimulated 45Ca2+ influx in A7r5 vascular smooth muscle cells induced either by receptor activation with [Arg]8 vasopressin or by the SR-Ca(2+)-ATPase inhibitor thapsigargin was increased more than threefold if cells were preloaded with the intracellular calcium chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid). The increased influx is probably due to an attenuation of negative feedback of Ca2+ on its own entry accompanied by increased Ca2+ storage capacity of BAPTA-loaded cells leading to diminished cellular Ca2+ release. We propose that BAPTA preloading could be a useful approach to investigate receptor-induced Ca2+ influx.
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