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  • Title: Determination of salivary cortisol by ELISA and its application to the assessment of the circadian rhythm in children.
    Author: Shimada M, Takahashi K, Ohkawa T, Segawa M, Higurashi M.
    Journal: Horm Res; 1995; 44(5):213-7. PubMed ID: 8582713.
    Abstract:
    In 35 young children, circadian rhythms of salivary cortisol levels were determined by ELISA using a commercially available kit with a minor modification. The concentration of labeled cortisol in the serum kit was reduced in order to measure cortisol in 10 microliters of saliva. Intra- and interassay coefficients of variation for salivary cortisol ranged from 2.4 to 9.9 and 3.2 to 8.9%, respectively. Recovery of salivary cortisol was 82.9-107.0%. There was a highly significant correlation between cortisol levels in saliva and serum in adults (r = 0.857). Salivary cortisol levels ranged from 0.01 to 2.252 micrograms/100 ml and showed significant diurnal variation in the children. Our ELISA is a precise, simple, noninvasive and useful method for clinical practice and study in infants and children.
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