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  • Title: Studies on the chemistry of human platelet factor 4.
    Author: Morgan FJ, Chesterman CN, McGready JR, Begg GS.
    Journal: Haemostasis; 1977; 6(1):53-8. PubMed ID: 858559.
    Abstract:
    The chemical characteristics of several PF4 preparations have been examined by gel electrophoresis, amino acid analysis and NH2-terminal amino acid sequence determination. Preparations of PF4 from gel filtration and from affinity chromatography appeared identical. A single NH2-terminal sequence was determined as follows: NH2-Glu-Ala-Glu-Glu-Asp-Gly-Asp-Leu-Gin-SCMCys-Leu-SCMCys-Val-Lys-Thr-Thr-Ser-Gln-Val-Arg-. The molecular weight of the PF4 subunits, which seem to be identical, was 7,100 based on the recovery of NH2-terminal glutamic acid.
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