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  • Title: Analysis of glucan synthesis by Streptococcus mutans.
    Author: Kuramitsu HK, Smorawinska M, Nakano YJ, Shimamura A, Lis M.
    Journal: Dev Biol Stand; 1995; 85():303-7. PubMed ID: 8586194.
    Abstract:
    Glucan synthesis by Streptococcus mutans GS-5 has been analyzed by examining the regulation of expression and structure-function relationships of the glucosyltransferases. Primer extension analysis of the gtf genes has identified the putative promoter sequences for these genes and indicated that the -10 sequences are similar to the Escherichia coli consensus sequence. Site-directed mutagenesis as well as deletion analysis of the enzymes have identified amino acid residues as well as functional domains which play important roles in glucan synthesis.
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