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  • Title: [The isolation and purification of the protective antigen and the edema factor from the culture filtrate of Bacillus anthracis STI-1].
    Author: Rogov SN, Beznosov MV, Chugaĭ VL, Bashkova VA.
    Journal: Med Parazitol (Mosk); 1995; (4):35-8. PubMed ID: 8587516.
    Abstract:
    A rapid method for producing a protective antigen (PA) and edema factor (EF), components of anthrax toxin, is described. The specific features of the method are as follows: addition of a mixture of protease inhibitors to the culture fluid; simultaneous concentration on a fiber filter and adsorption on hydroxylapatite, followed by non-linear gradient a phosphate concentration; purification of elution of a phosphate concentration; purification of eluates from salts via electrodialysis. Resultant from protein desorption, PA and EF agents are electrophoretically homogeneous by no less than 80%, 10 liters of filtrate yielded 8 mg of PA and 5 mg of EF. The resultant agents were tested for their biological properties and protective activity.
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