These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Expression of the isopentenyl transferase gene is regulated by auxin in transgenic tobacco tissues.
    Author: Zhang XD, Letham DS, Zhang R, Higgins TJ.
    Journal: Transgenic Res; 1996 Jan; 5(1):57-65. PubMed ID: 8589740.
    Abstract:
    The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. The addition of auxin to the media rapidly reduced the level of cytokinins in the transgenic tissues and this was associated with a reduction in IPT mRNA and protein levels. It is concluded that the hormone auxin can regulate expression of a gene involved in biosynthesis of the second hormone cytokinin. Although exogenous benzyladenine did not directly affect ipt gene expression, it did antagonize the effect of auxin on levels of cytokinins and IPT mRNA and protein.
    [Abstract] [Full Text] [Related] [New Search]