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  • Title: Experimental bias in the evaluation of the cellular transient expression in DNA co-transfection experiments.
    Author: Bergeron D, Barbeau B, Léger C, Rassart E.
    Journal: Cell Mol Biol Res; 1995; 41(3):155-9. PubMed ID: 8589755.
    Abstract:
    beta-Galactosidase (beta-gal) expressing vectors are commonly used to standardize the transfection efficiency in transient expression experiments. In the Chinese hamster ovary (CHO) cell line, we transfected beta-gal expressing vectors in combination with different plasmid DNAs. We reported here that the presence of specific DNAs led to statistically significant variations in the beta-gal expression level. Therefore, the measure of beta-gal activity is not necessarily an accurate method to monitor transfection efficiency, and its use to normalize the expression from reporter genes could be questionable.
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