These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Determination of beta-(1-3),(1-4)-D-glucans in barley by reversed-phase high-performance liquid chromatography.
    Author: Pérez-Vendrell AM, Guasch J, Francesch M, Molina-Cano JL, Brufau J.
    Journal: J Chromatogr A; 1995 Dec 22; 718(2):291-7. PubMed ID: 8589819.
    Abstract:
    An HPLC method for the determination of beta-glucan in barley was developed. The beta-glucan was hydrolysed with lichenase [endo-beta-(1-3),(1-4)-D-glucan-4-glucanhydrolase from Bacillus subtilis] to oligosaccharides, which were analysed by reversed-phase HPLC using water as the mobile phase at a flow-rate of 0.7 ml/min. The separation of the oligosaccharides was performed in a C18 stainless-steel column (Spherisorb ODS-2) with 5-microns particles in less than 10 min, with a refractive index detection.
    [Abstract] [Full Text] [Related] [New Search]