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  • Title: Use of digoxigenin-labelled probes for the detection of B19 parvovirus DNA in batches of blood products.
    Author: Lefrère JJ, Mariotti M.
    Journal: Cell Mol Biol (Noisy-le-grand); 1995 Nov; 41(7):985-8. PubMed ID: 8595378.
    Abstract:
    Being thermostable and non lipid-enveloped, the B19 parvovirus cannot be destroyed by the chemical and physical treatments presently used to inactivate lipid-enveloped viruses such as HIV, hepatitis C and B viruses in plasma derivatives. A polymerase chain reaction assay was used to detect B19 parvovirus DNA. Amplified products were detected through a non-radioactive technology based on the use of a digoxigenin-labelled probe. B19 parvovirus DNA was detected in 20% of the studied batches of factor coagulation concentrates and in none of the albumin batches.
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