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  • Title: Receptors for atrial natriuretic peptide in adrenal chromaffin cells.
    Author: Niina H, Kobayashi H, Yamamoto R, Yuhi T, Yanagita T, Wada A.
    Journal: Biochem Pharmacol; 1996 Mar 22; 51(6):855-8. PubMed ID: 8602882.
    Abstract:
    Receptors for atrial natriuretic peptide (ANP) in isolated bovine adrenal chromaffin cells were characterized. 125 I-ANP specifically bound to the cells with a Kd of 103 pM and a Bmax of 5.6 fmol/10(6) cells (16.5 fmol/mg of cell protein). C-ANF, a highly selective ligand for ANP-C receptors of natriuretic peptides, did not compete for 125 I-ANP binding at concentrations up to 10nM. Chemical cross-linking of 125I-ANP to the cells showed a single molecular size of the 120 kDa binding site on SDS gel electrophoresis under reducing conditions. CNP, a specific peptide for the ANP-B receptor, was much less potent than ANP in inhibiting 125 I-ANP binding and in displacing 125 I-ANP from the 120 kDa band. These results suggest that ANP specifically binds to the ANP-A receptor of 120 kDa and that there is no ANP-C receptor in bovine adrenal chromaffin cells.
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