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  • Title: [Expression of a gene encoding the porcine rotavirus VP7 capsid using a nondefective recombinant adenoviral genome].
    Author: Doronin KK, Iurov GK, Grinenko NF, Zakharchuk AN, Denisova TS, Baburina TM, Krugliak VA, Naroditskiĭ BS.
    Journal: Mol Gen Mikrobiol Virusol; 1995; (4):35-9. PubMed ID: 8604234.
    Abstract:
    A nondefective recombinant human adenovirus 5 (Ad5) carrying the gene encoding for the porcine rotavirus outer capsid protein VP7 in the E3 region of the Ad5 genome has been obtained. mRNA for VP7 in recombinant Ad5KV14/VP7 infected cells was demonstrated by means of RT-PCR. Radioimmunoprecipitation of cell extracts infected with Ad5KV14/VP7 on early (12 h p.i.) and late (24 h p.i.) stages of adenovirus infection with rabbit polyclonal antiserum raised to purified rotavirus virions revealed a recombinant protein possessing the same electrophoretic mobility, 37 kDa, as the native rotavirus K VP7. One-dimensional peptide mapping also demonstrated the identity of the recombinant and native VP7.
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