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  • Title: Isolation of prolactin from human amniotic fluid.
    Author: Rathnam P, Cederqvist L, Saxena BB.
    Journal: Biochim Biophys Acta; 1977 May 27; 492(1):186-93. PubMed ID: 861247.
    Abstract:
    Amniotic fluid, obtained from women at term, was centrifuged and concentrated by ultrafiltration. The concentrated amniotic fluid was purified by gel filtration on Sephadex G-100 and ion-exchanged chromatography on DEAE-cellulose. The fractions obtained during purification procedures were assayed for prolactin and somatotropin activities by respective radioimmunoassays. The prolactin-rich fraction obtained from ion-exchange chromatography was further purified by isoelectric focusing. A yield of 0.21 mg of highly pruified prolactin containing 40 units/mg was obtained from 1 liter of the amniotic fluid. The prolactin was free of somatotropin and human placental lactogen.
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