These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Interleukin-1 receptor antagonist inhibits subcutaneous B16 melanoma growth in vivo.
    Author: McKenzie RC, Oran A, Dinarello CA, Sauder DN.
    Journal: Anticancer Res; 1996; 16(1):437-41. PubMed ID: 8615650.
    Abstract:
    BACKGROUND: Previously we demonstrated that injection of Interleukin-alpha (IL-1) stimulated B16 melanoma tumour growth in vivo, associated with increased intercellular adhesion molecule-1 (ICAM-1) expression on the tumour cells (Photodermatol Photoimmunol Photomed 1994; 10: 74-79, ). METHODS: In the present study, we examined the effect of blocking IL-1 receptors - by addition of recombinant Interleukin-1 receptor antagonist (IL-1RA) - on melanoma tumour growth in vivo and in vitro. RESULTS: Subcutaneous co-injection of IL-1RA with B16 tumour cells into C57BL/6 mice, followed by injection of IL-1RA every second day reduced tumour growth significantly from day 18 to day 33 post-injection. Treatment with IL-1RA appeared to have a bi-phasic effect, low doses being more effective than dosed > 1 microgram/mouse. After 33 days, mice treated with 1.0 or 0.1 micrograms/2nd day had significantly smaller tumours than controls (p < 0.05), however, mice treated with 10 micrograms had tumours no different in size from those of untreated controls. However, survival of IL-RA-treated mice was not significantly different between treatment groups. Addition of IL-1RA to B16 cultures in vitro caused a small dose-dependent decrease in cell growth. Maximum inhibition (64% of control numbers) was observed after 48h in media containing > or = 10 ng/ml Il-1RA (p < 0.05). Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), we examined the expression of ICAM-1 message in B16 cells treated in culture with 10 ng/ml IL-1RA or 10 ng/ml IL-1 alpha for 6h or 24h. In IL-1-treated cultures ICAM-1 mRNA expression was increased to 161% of control levels. After 24h, ICAM-1 message was 198% control levels (p = 0.0008). Treatment with IL-1RA reduced the constitutive ICAM-1 expression to 75% of basal after 6h and to 68% of basal after 24h 9 (p = 0.011). CONCLUSION: Abrogation of IL-1 activity, in combination with other systemic therapies may prove useful in the treatment of melanoma.
    [Abstract] [Full Text] [Related] [New Search]